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Addgene inc elongin c
Elongin C, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/elongin+c/V21+CS2%2B+RFPbb+(Plasmid+%2317112)/pmc12616682__ja5c10354_si_001-14-23-30
Average 93 stars, based on 22 article reviews
elongin c - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

Plasmid Preparation:

Article Title: Development of a BCL-xL and BCL-2 dual degrader with improved anti-leukemic activity.
Article Snippet: .. The plasmid co-expresses Elongin B and Elongin C (AA 17-112) (EloB/C) was a gift from Nicola Burgess-Brown (Addgene plasmid # 110274). pGEX-6P-1-VHL(54-213) (pDL2200) was constructed through Gibson assembly method. .. The primer pair (5′-CTGGAAGTTCTGTTCCAGGGGCCCATGGAGGCCGGGCGGCCGCGG C-3′ and 5′-ATCGTCAGTCAGTCACGATGCGGCTCAATCTCCCATCCGTTG ATGTGCAATG-3′) and HA-VHL (a gift from William Kaelin, Addgene plasmid # 19999) were used to clone the VHL(54-213)-containing fragment.

Article Title: Development and crystal structures of a potent second-generation dual degrader of BCL-2 and BCL-xL.
Article Snippet: .. Elongin B (full-length) and Elongin C (aa 17-112) co-expression pACYCDuet1 plasmid was purchased from Addgene (Addgene plasmid # 110274). cDNA of VHL (aa 54-213) was cloned into pET28a with thrombin cleavable N-terminal 6xHis tag using Gibson Assembly. ..

Article Title: Development and crystal structures of a potent second-generation dual degrader of BCL-2 and BCL-xL
Article Snippet: .. Elongin B (full-length) and Elongin C (aa 17-112) co-expression pACYC-Duet1 plasmid was purchased from Addgene (Addgene plasmid # 110274). cDNA of VHL (aa 54-213) was cloned into pET28a with thrombin cleavable N-terminal 6xHis tag using Gibson Assembly. ..

Construct:

Article Title: Development of a BCL-xL and BCL-2 dual degrader with improved anti-leukemic activity.
Article Snippet: .. The plasmid co-expresses Elongin B and Elongin C (AA 17-112) (EloB/C) was a gift from Nicola Burgess-Brown (Addgene plasmid # 110274). pGEX-6P-1-VHL(54-213) (pDL2200) was constructed through Gibson assembly method. .. The primer pair (5′-CTGGAAGTTCTGTTCCAGGGGCCCATGGAGGCCGGGCGGCCGCGG C-3′ and 5′-ATCGTCAGTCAGTCACGATGCGGCTCAATCTCCCATCCGTTG ATGTGCAATG-3′) and HA-VHL (a gift from William Kaelin, Addgene plasmid # 19999) were used to clone the VHL(54-213)-containing fragment.

Purification:

Article Title: Identification of a Highly Cooperative PROTAC Degrader Targeting GTP-Loaded KRAS(On) Alleles
Article Snippet: .. The VCB complex was expressed and purified as described previously.1 Biotinylated VCB was also produced as previously described.2 Briefly, N-terminally His6-tagged VHL (54-213), Elongin C (17-112) and Elongin B (1-104) (Addgene ID 204500 & 204501) were co-expressed in E. coli and the complex isolated by Ni-a inity chromatography. ..

Produced:

Article Title: Identification of a Highly Cooperative PROTAC Degrader Targeting GTP-Loaded KRAS(On) Alleles
Article Snippet: .. The VCB complex was expressed and purified as described previously.1 Biotinylated VCB was also produced as previously described.2 Briefly, N-terminally His6-tagged VHL (54-213), Elongin C (17-112) and Elongin B (1-104) (Addgene ID 204500 & 204501) were co-expressed in E. coli and the complex isolated by Ni-a inity chromatography. ..

Isolation:

Article Title: Identification of a Highly Cooperative PROTAC Degrader Targeting GTP-Loaded KRAS(On) Alleles
Article Snippet: .. The VCB complex was expressed and purified as described previously.1 Biotinylated VCB was also produced as previously described.2 Briefly, N-terminally His6-tagged VHL (54-213), Elongin C (17-112) and Elongin B (1-104) (Addgene ID 204500 & 204501) were co-expressed in E. coli and the complex isolated by Ni-a inity chromatography. ..

Chromatography:

Article Title: Identification of a Highly Cooperative PROTAC Degrader Targeting GTP-Loaded KRAS(On) Alleles
Article Snippet: .. The VCB complex was expressed and purified as described previously.1 Biotinylated VCB was also produced as previously described.2 Briefly, N-terminally His6-tagged VHL (54-213), Elongin C (17-112) and Elongin B (1-104) (Addgene ID 204500 & 204501) were co-expressed in E. coli and the complex isolated by Ni-a inity chromatography. ..

Clone Assay:

Article Title: Development and crystal structures of a potent second-generation dual degrader of BCL-2 and BCL-xL.
Article Snippet: .. Elongin B (full-length) and Elongin C (aa 17-112) co-expression pACYCDuet1 plasmid was purchased from Addgene (Addgene plasmid # 110274). cDNA of VHL (aa 54-213) was cloned into pET28a with thrombin cleavable N-terminal 6xHis tag using Gibson Assembly. ..

Article Title: Development and crystal structures of a potent second-generation dual degrader of BCL-2 and BCL-xL
Article Snippet: .. Elongin B (full-length) and Elongin C (aa 17-112) co-expression pACYC-Duet1 plasmid was purchased from Addgene (Addgene plasmid # 110274). cDNA of VHL (aa 54-213) was cloned into pET28a with thrombin cleavable N-terminal 6xHis tag using Gibson Assembly. ..



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Image Search Results


Journal: iScience

Article Title: Discovery of SOCS7 as a versatile E3 ligase for protein-based degraders

doi: 10.1016/j.isci.2024.109802

Figure Lengend Snippet:

Article Snippet: Elongin-C rabbit antibody , Abcam , Cat#ab226831.

Techniques: Recombinant, Transfection, Magnetic Beads, Fluorescence, Expressing, Software, Sterility, Suction Filtration